Part:BBa_K4350815:Design

N. nambi Caffeylpyruvate Hydrolase (nnCPH)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 901
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This sequence is codon-optimized for expression in the model cyanobacterial organism, Synechocystis sp. PCC 6803. Additionally, illegal restriction sites have been removed for compatibility with the MoClo golden gate cloning assembly standard.

Source

The sequence for this enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115.